Carbolfuchsin solution kinyoun, for staining acidfast. Kinyoun, is a procedure used to stain acidfast species of the bacterial genera mycobacterium and nocardia and the apicomplexan genus cryptosporidium. Used in the demonstration of acidfast bacteria belonging to the genus mycobacterium, which include the causative agent for tuberculosis. This file is licensed under the creative commons attributionshare alike 4.
Suggested by haawk for a 3rd party monetize your music today. Tincion simple y tincion diferencial free download pdf ebook. Our product line consists of chemical solutions prepared to exact quality standards and certified for use in laboratories and production processes. Phenol is employed to render the cell walls of bacteria permeable to the fuchsin stain. At the age of 16, he studied medicine with his father, john hendricks kinyoun, who was a general practitioner. The lipoid capsule of the acidfast organism takes up carbolfuchsin and resists decolorization with a dilute acid rinse. Murray pr, baron e, pfaller m, tenover f, yolken, eds. The use of weak acid for differentiation allows excess stain to be removed from tissues, but will not remove stain from the acidfast organisms. If the pdf does not display below, you may also download it. Kinyoun staining method uses carbolfuchsin as a primary stain, followed by decolorization with an acidalcohol solution and methylene blue as a counterstain. Kinyoun carbolfuschsin has a greater concentration of phenol and basic fuchsin and does not require heating in order to stain properly.
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